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Application: Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.
Background: KAT6B, or Lysine Acetyltransferase 6B, also known as MORF, is a histone acetyltransferase of the MYST family and plays a crucial role in chromatin organization and gene expression regulation by acetylating histones, which impacts DNA accessibility and transcription. It is part of the MOZ/MORF protein complex. KAT6B is involved in various biological processes, including brain development and RUNX2 (Runt-related transcription factor 2)-dependent transcriptional activation 1. Mutations in the KAT6B gene are associated with several rare genetic disorders, primarily affecting neurodevelopment. It is also linked to cancer, with its role as tumor suppressor or oncogene being type dependent. Inhibitors of KAT6A and KAT6B have been developed to block acetylation activity, showing promising clinical trial results in slowing tumor progression of breast cancer patients.
Contraindications: This assay kit is compatible with up to 1% final DMSO concentration.
Description: The KAT6B (MORF) Chemiluminescent Assay Kit is an ELISA-based assay designed to measure the histone acetyl transferase activity of KAT6B (lysine acetyltransferase 6B, also known as MORF) for screening and profiling applications. This kit comes in a convenient 96-well format with enough purified KAT6B (amino acids 710-1020), acetyl donor, pre-coated plate with histone substrate, all the reagents necessary for assay detection, and blocking buffer for 96 enzyme reactions.Figure 1. KAT6B (MORF) Chemiluminescent Assay Kit schematic. KAT6B protein is added to a plate pre-coated with a histone substrate in the presence of acetyl-CoA. After washing, an anti-acetyl-lysine antibody is added, followed by a secondary HRP-antibody. Lastly, HRP substrate is added and the chemiluminescence signal generated can be measured. The signal is proportional to the acetyltransferase activity of KAT6B.Need us to run inhibitor screens or profile your compounds against KAT1? Check out our Acethyltransferase Screening &Profiling Services.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This kit comes in a convenient 96-well format with enough purified KAT6B (amino acids 710-1020), acetyl donor, pre-coated plate with histone substrate, all the reagents necessary for assay detection, and blocking buffer for 96 enzyme reactions.
Target: KAT6B
Uniprot: Q8WYB5
Unit Definition: Assay Kit
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Jeselsohn R., and Polyak K., et al., 2023 Cell Chemical Biology 30 (10): P1183-1185.Wiesel-Motiuk N. and Assaraf Y., 2020 Drug Resistance Updates 53:100729.
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