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Encompassing Amino Acids: 2-186
Application: Useful for KRAS(G12D) GDP/GTP nucleotide exchange reaction.
Assay Conditions: Assay was performed by following the KRAS Nucleotide Exchange Assay Kit (BPS Bioscience, #79859) protocols with BODIPY-GDP-loaded KRAS(G12D) concentrated at 0.12 mg/ml and GTP titrated 3-fold from 100 µM - 0 µM.
Description: Recombinant human KRAS (GTPase), isoform a, encompassing amino acids 2-186 (end). In this construct the N-terminal end of the protein contains a His-tag (6xHis) followed by an optimal TEV protease target sequence that allows cleavage of the His-tag from the protein (TEV: tobacco etch virus cysteine protease BPS Bioscience #50308). The protein also contains mutation of interest G12D. The protein was affinity purified and was loaded with BODIPY-GDP. Unbound BODIPY-GDP was removed by spin column and the protein is ready for a GDP/GTP exchange assay
Format: Aqueous buffer solution
Formulation: 20 mM HEPES, pH 7.4, 150 mM NaCl and 1 mM DTT
Genbank: NM_033360
Purity: ≥90%
Storage Stability: Aqueous buffer solution.
Tags: N-terminal His-tag
Uniprot: P01116
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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