Background: The antibody against Laminin primarily labels basal membranes (especially s. c. lamina lucida) and may be used to detect disturbances in the basal membrane during tumour invasion. Laminin is also found in extracellular matrix not related to basal membranes, e.g. in the proximity of neurons especially during development, regeneration and tumour development. Laminins are glycoproteins of the extracellular matrix, especially of the basal membranes. They are multi-functional molecules which play an important role during development and differentiation of organs and for cell movements. These properties rely on the interaction of laminins with receptors on cell surfaces like integrins, collagen IV, proteoglycans. Laminins consist of 2 heavy chains (A and M, 300-400 kDa) and 1 light chain (B1, B2 or S, 200 - 220 kDa). Murine laminin 100%, murine collagen type I, II, III, IV < 0.1%, murine fibronectin < 0.1% (RIA at 1:40000 dilution).
Positive Control: Mouse skin.
Immunogen: Purified laminin from murine tumour tissue.
Purification Method: Affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!.
Concentration: app. 1 mg/ml.
References: See list of references in BIOLOGO ECM brochure.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.
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