Clone: A103.
Background: The antibody A103 is suitable for the detection of Melan-A antigen (syn. MART-1) in cells of melanoic origin. The antibody will label 80-90% of metastasing malign melanoma (1, 6). Melan-A-negative melanoma usually are of the spindle cell or desmoblastic type. The antibody also reacts positive with angiomyolipoma (4) and steroid hormone producing cells of the adrenal gland, ovary, testis and especially with carcinoma of these organs. Melan-A is a melanoma specific antigen, which is detected by cytotoxic T-cells. It is exprimed by benign naevi and melanoma cells. 20-22 kDA Melan-A protein, no cross-reactivity to MAGE-1 and Tyrosinase.
Positive Control: Melanoma.
Immunogen: Recombinant Melan-A protein.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
References: 1. Jungbluth AA, Busam KJ, Gerald WL, Stockert E, Coplan KA, Iversen K, MacGregor DP, Old LJ, Chen YT. A103: An anti-melan-a monoclonal antibody for the detection of malignant melanoma in paraffin-embedded tissues. Am J Surg Pathol. 1998 May;22(5):595-602. 2.Berset M, Cerottini JP, Guggisberg D, Romero P, Burri F, Rimoldi D, Panizzon RG. Expression of Melan-A/MART-1 antigen as a prognostic factor in primary cutaneous melanoma. Int J Cancer. 2001 Jan 20;95(1):73-7. 3. Florell SR, Zone JJ, Gerwels JW. Basal cell carcinomas are populated by melanocytes and Langerhan's cells. Am J Dermatopathol. 2001 Feb;23(1):24-8. 4. Zavala-Pompa A, Folpe AL, Jimenez RE, Lim SD, Cohen C, Eble JN, Amin MB. Immunohistochemical study of microphthalmia transcription factor and tyrosinase in angiomyolipoma of the kidney, renal cell carcinoma, and renal and retroperitoneal sarcomas: comparative evaluation with traditional diagnostic markers. Am J Surg Pathol. 2001 Jan;25(1):65-70. 5. Schmuth M, Spotl L, Zelger B, Weinlich G, Zelger B. Clear cells in acral melanoma. Eur J Dermatol. 2001 Jan-Feb;11(1):21-4.
UniProt: Q16655.
Caution: *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
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