Clone Number: 4C5
Immunogen: fragments human tonsil
Concentration: 60 ug/ml
Storage buffer: serum with 0.1% sodium azide
Additional info: A BALB/c mouse was immunized subcutaneously in its footpads with fragments of a human tonsil. After fusing the lymphocytes from the popliteal lymph nodes of this mouse with murine SP2/0 myeloma cells, the antibody producing cells were selected on their immunohistochemical staining pattern. Later on the antibody was biochemically analysed and revealed to recognize the CD21 molecule (140kD) by immunoprecipitation procedures. This antibody reacts with the CD21 (140kD) molecule, expressed (moderate) on mature B-cells and (at high density) on follicular dendritic cells (FDC).
Applications: IHC-fr (1:20-1:50), IF, FC (1:50)
References: Abo T et al. J of Immunol 1981; 127: 1024/Jaspars et al. Histopathol 1994
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