Description: MAb VU-11D1 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of MAb VU-11D1 is the 7-mer TSAPDTR of the MUC1 tandem repeat as established with 'epitope fingerprinting'. The'extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: VU-11D1
Immunogen: Human breast cancer cell line ZR-75 cells
Antibody Reactivity: CA15-3/CD227/EMA/MUC1
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
Mol Cell Proteomics (2009) 8(7), 1697-1707. (ELISA)
Entrez Gene ID: 4582
Expected AB Applications: ELISA (published for clone)
Z-Antibody Applications: ELISA (published)
Antibody Application Notes: ELISA: For coating, order antibody without BSA; Optimal dilution for a specific application should be determined./Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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