Shelf life (days): 365
Info: Cayman’s MPO Inhibitor Screening Assay Kit provides convenient fluorometric methods for screening inhibitors to both the chlorination and peroxidation activities of MPO. The chlorination assay utilizes a non-fluorescent thiomorphiline-based probe (NBD-TM), which is selectively cleaved by hypochlorite (-OCl) to yield the highly fluorescent compound, NBD.{71274} Fluorescence intensity is analyzed using an excitation wavelength of 500 nm and an emision wavelength of 550 nm. The peroxidation assay utilizes the peroxidase component of MPO, where a single two-electron oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one-electron reductions back to native enzyme by compound II (MPO-II).{16905} The reaction between hydrogen peroxide and 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed using an excitation wavelength of 530 nm and an emission wavelength of 590 nm.
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