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Encompassing Amino Acids: full length
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na orthovanadate, 0.5 mM ATP, 0.3 mg/ml MBP substrate, and 0.2 µg/ml NEK2.
Description: Human NEK2 (GenBank Accession No. NM_002497) full length with N-terminal His-tag, MW=53 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.05% Tween-20, 40% glycerol, and 3 mM DTT.
Genbank: NM_002497
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P51955
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of myelin basic protein (MBP) substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Fletcher, L. et al., Biochim. Biophys. Acta 1744(2):89-92 (2005).
2. Fardilha, M. et al., Ann. N.Y. Acad. Sci. 1030:468-478 (2004).Application Reference:
Nek2 phosphorylates and stabilizes β-catenin at mitotic centrosomes downstream of Plk1 (2014)
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