Clone: R711
Specificity: Human NGF. Has cross-reactivity with Mouse ß-NGF in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered histidine and arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH 6.0
Immunogen: Recombinant Human NGF / NGFB protein
Gene: NGF
Description: NGF antibody LS-C820662 is an unconjugated rabbit monoclonal antibody to human NGF. Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA which immobilized recombinant Human ß-NGF at 2ug/ml (100 µL/well) in the plate, the Rabbit anti-Human ß-NGF Monoclonal Antibody can block the binding of 5 ug/ml of biotinylated Human NTRK1/Fc Chimera Protein to human ß-NGF, the EC50 is 3. 84 ug/ml. Neutralization: The neutralization activity of antibody is Measured by its ability to neutralize ßNGFinduced proliferation in the TF1 human erythroleukemic cell line. The Neutralization titer (IC50) is typically 0.0480.19 µg/mL in the presence of 10 ng/mL Recombinant Human ßNGF. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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