Clone: RC6
Specificity: Human NGF. Has cross-reactivity with Mouse ß-NGF in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered solution in PBS
Immunogen: Recombinant Human NGF / NGFB protein
Gene: NGF
Description: NGF antibody LS-C820663 is an unconjugated rabbit monoclonal antibody to human NGF. Validated for Block, ELISA and Neut.
Usage: Block: In a functional ELISA which immobilized recombinant Human ß-NGF at 2 ug/ml (100 µL/well) in the plate, the Rabbit Anti-Human ß-NGF Monoclonal Antibody can block the binding of 1 ug/ml of biotinylated Human NTRK1 Protein to human ß-NGF, the EC50 is 0.90 ug/ml. Neutralization: The neutralization activity of ß-NGF Neutralizing Antibody is Measured by its ability to neutralize ß-NGF induced proliferation in the TF-1 human erythroleukemic cell line. The Neutralization titer (IC50) is typically 17-70 ng/mL in the presence of 220 ng/mL Recombinant Human ß-NGF. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Concentration: 1.7 mg/ml
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