Description: By immunohistochemistry, this antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: NKI-beteb
Immunogen: Membranes from a human melanoma metastasis
Antibody Reactivity: gp100/Pmel17/SILV
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Am J Pathol (1988) 130(1): 179. (mAb characterization; IHC, FFPE; IF; ELISA; IP; WB)
- Am J Pathol (1993) 143(6): 1579. (IHC, frozen; IF)
- J Biol Chem (2004) 279(27): 28330-28338. (epitope mapping; IP; reports that NKI-beteb does not work well for WB)
- Exp Dermatol (2010) 19(8): e282-e285. (IF; Flow, intracellular)
Entrez Gene ID: 6490
Expected AB Applications: ELISA (published for clone)/Flow (intracellular) (published for clone)/IHC (frozen) (published for clone)/IF (published for clone)/IP (published for clone)
Z-Antibody Applications: Flow, intracellular (published)/IF (published)/IHC, frozen (published)/ELISA (published)/IHC, FFPE (verified)/IP (published)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Immunohistology formalin-fixed 1-2 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Immunofluorescence 1-2 ug/mL/Does not react with rat, others not tested/Optimal dilution for a specific application should be determined by user
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