Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 1-314(end)
Application: Useful for deglycosylation.
Assay Conditions: Assay run according to the PNGase F Deglycosylation Kit (BPS Bioscience #82864), following the protocol using denaturing conditions.
Background: PNGase F, also known as peptide-N-Glycosidade F, is an amidase that is widely used to remove N-glycans from glycoproteins and glycopeptides. N-linked glycans are sugar chains attached to asparagine residues of proteins. PNGase works by cleaving between the innermost GlcNAc and asparagine (not when the GlcNAC is linked to a α1-3 fucose). The glycan-cleaving activity of PNGase F is useful for studying underlying protein structure and glycoprotein behavior in the fields of proteomics, pharmaceutical research, and immunology.
Biological Activity: 500 Units/µlUnit Definition: One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 µl.
Description: Recombinant E. meningoseptica PNGase F (Peptide-N-Glycosidase F) full-length, encompassing amino acids 1-314(end). This construct contains a C-terminal His-tag (6x His) and was affinity purified.
Format: Aqueous buffer solution
Formulation: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, and 50% Glycerol
Genbank: AF165910
Purity: ≥90%
Storage Stability: At least 6 months at –80°C.
Tags: His
Target: PNGase F
Uniprot: Q9XBM8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
English
Deutsch
