Immunogen: Synthetic peptide from the C-terminus of human VAChT (11 amino acid residues) conjugated to BSA
Concentration: n/a
Storage buffer: Lyophilized; reconstitute in 100 µl dist. water
Additional info: The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholinesterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes staining.Positive control: frozen sections of human small intestine (Stefanini fixation).
Applications: IHC-fr (1:800-1:1,200)