QuicKey Pro Human PⅢNP (N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit

Storage: 2-8°C，6 months

Target Synonym: P3NP;N-Propeptide Of Type Ⅲ Procollagen;Procollagen Ⅲ Amino Terminal Propeptide

Usage: This ELISA kit applies to the in vitro quantitative determination of Human PⅢNP concentrations in serum, plasma and other biological fluids.

Tested Sample Types: Serum, plasma and other biological fluids

Type: Sandwich-ELISA

Assay Time: 1 h 30 min

Detection Type: Colorimetric method;ELISA;Sandwich

Sensitivity: 0.09 ng/mL

Detection Range: 0.16-10 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PⅢNP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PⅢNP are added to the micro ELISA plate wells. Human PⅢNP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PⅢNP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PⅢNP. The concentration of Human PⅢNP in the samples is then determined by comparing the OD of the samples to the standard curve.

• SKU: ELSE-OSEL-H0033-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-OSEL-H0033-48
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA