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Application: Useful for the study of TDO enzymology, inhibitor screening, and selectivity profiling.
Background: L-tryptophan (L-Trp) is an essential amino acid necessary for proteinsynthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established asa key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains animmunosuppressive microenvironment by starving immune cells of L-Trp and releasingdegradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3dioxygenase (TDO), a rate limiting enzymes in this pathway, is upregulated in many tumors,providing cancer cells with an avenue for immune evasion.
Contraindications: DMSO >0.5%, strong acids or bases, ionic detergents, high salt.Warning: the Fluorescence Solution contains a component that is known to be a skin and eyeirritant. Use caution and appropriate personal protective equipment when handling thiscomponent.
Description: The TDO2 Fluorogenic Inhibitor Screening Assay Kit – 384 (Human) is designed to measure TDO2 (tryptophan 2,3-dioxygenase 2) activity for screening and profiling applications. The assay kit comes in a convenient 384-well format, with enough recombinant TDO2, fluorogenic substrate and solution, and assay buffer for 400 enzyme reactions. TDO2 converts the conversion of tryptophan to N-formylkynurenine (NFK), which can react with the TDO Fluorogenic reagent present in the TDO Fluorogenic Reaction Solution and generate a green fluorescent molecule. The increase in fluorescence is proportional to TDO2 activity.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The kit comes in a convenient format, with enough reaction solution and enzyme to perform a total of 400 reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Seegers, N., et al. J. Biomol. Screen. 2014. 19(9):1266-74.
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