Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) (P-GF.44C), CF740 conjugate, 0.1mg/mL

Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) (P-GF.44C), CF740 conjugate, 0.1mg/mL
SKU
BTMBNC741244-500
Packaging Unit
500 uL
Manufacturer
Biotium

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Description: This antibody recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: P-GF.44C

Immunogen: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Antibody Reactivity: PD-ECGF/Thymidine Phosphorylase

Entrez Gene ID: 1890

Z-Antibody Applications: IHC, FFPE (verified)/WB (verified)

Verified AB Applications: IHC (FFPE) (verified)/WB (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunohistology (formalin) 1-2 ug/mL/Western blotting 0.5-1 ug/mL Immunoprecipitation 0.5-1 ug/500ug protein lysate/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min/Optimal dilution for a specific application should be determined by user
More Information
SKU BTMBNC741244-500
Manufacturer Biotium
Manufacturer SKU BNC741244-500
Package Unit 500 uL
Quantity Unit STK
Reactivity Human, Mouse (Murine), Rat (Rattus)
Clonality Monoclonal
Application Western Blotting, Immunohistochemistry
Isotype IgG1
Host Mouse
Conjugate Conjugated, CF740
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