Clone: R101
Specificity: Human TNF-alpha. Has cross-reactivity with Cynomolgus TNFa/Canine TNFa, and Ferret TNFa. No cross-reactivity with Mouse TNFa, or Rat TNFa in ELISA assay
Antibody Modification: Preservative Free Unconjugated
Presentation: 0.2 µm filtered solution in PBS
Immunogen: Recombinant Human TNF-alpha / TNFA Protein
Gene: TNF Alpha
Description: TNF Alpha antibody LS-C820676 is an unconjugated rabbit monoclonal antibody to human TNF Alpha. Validated for Block, Flow and Neut.
Usage: Block: In a functional ELISA which immobilized recombinant Human TNFR1 at 1ug/ml (100 µL/well) in the plate, the Rabbit Anti-Human TNFa Monoclonal Antibody can block the binding of 0.2 ug/ml of biotinylated Human TNFa to human TNFR1, the EC50 is 1. 25 ug/ml;In a functional ELISA which immobilized recombinant Human TNFR2 at 2 ug/ml (100 µL/well) in the plate, the Rabbit Anti-Human TNFa Monoclonal Antibody can block the binding of 0.2 ug/ml of biotinylated Human TNFa to human TNFR1, the EC50 is 1. 26 ug/ml. Neutralization: The neutralization activity of TNFa Neutralizing Antibody is Measured by its ability to neutralize TNF-a induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 8. 8-35. 2 ng/mL in the presence of 2 ng/mL Recombinant Human TNF-a and 1 ug/ml actinomycin D. Please Note: Optimal concentrations/dilutions should be determined by the end user.
Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
Recommended Storage: Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Concentration: 3.69 µg/µl
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