Shelf life (days): 730.0
Assay Range: 0.19-25 ng/ml
Sensitivity: 80% B/B0: 0.5 ng/ml
Cross Reactivity: 25-OH Vitamin D3 100%; 1,25-(OH)2 Vitamin D2 265%; 25-OH Vitamin D2 200%; 1,25-(OH)2 Vitamin D3 100%; 24,25-(OH)2 Vitamin D2 14%; 24,25-(OH)2 Vitamin D3 4.2%; Vitamin D2 2.8%; Vitamin D3 <0.01%
Formula Weight: 0.0
Notes: The primary synthesis of functional vitamin D begins in the skin, where a cholesterol by-product acted upon by UV light forms vitamin D3. Vitamin D3 is carried through the bloodstream, attached to the vitamin D binding protein, to the liver where it is converted to 25-hydroxy vitamin D3 (25(OH)D3). This product is then transported to the kidney where it is further converted to 1,25-dihydroxycholecalciferol (1,25(OH)2D3), the biologically active form. Active 1,25(OH)2D3 is very short-lived and is rapidly metabolized to its deactivated forms. Vitamin D Immunoassay: Accurate Vitamin D measurement in plasma or serum Cayman’s Vitamin D ELISA Kit is a competitive assay that can be used for Vitamin D quantification in plasma or serum. Because of the short half-life of the biologically active 1,25(OH)D form, this Vitamin D assay primarily detects the more metabolically stable forms, 25(OH)D3 and 25(OH)D2. Accurate detection of these forms requires that they be displaced from the vitamin D binding protein prior to measurement. A simple, acetone-based purification protocol is included as the initial procedure of the Vitamin D assay kit for this purpose.
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