Shelf life (days): 1460.0
Formulation: A solid
Formal Name: 3',6'-bis(acetyloxy)-2',7'-dichloro-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid
Purity: ≥85% (mixture of isomers)
Formula Markup: C25H14Cl2O9
Formula Weight: 529.27866
CAS Number: 127770-45-0
Notes: 5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.{12088} Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production.