AAV1 Luciferase

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Applications: Use as a positive control for transductionOptimize transduction assays and track protein expression over time

Description: Adeno-Associated Virus Serotype 1 (AAV1) exhibits high homology with other AAV serotypes. AAV1 efficiently transduces muscle tissue, as determined by a region of the capsid protein VP1 (amino acids 350 to 430) which functions as a major determinant of tissue tropism.These AAV particles constitutively express the firefly (Photinus pyralis) luciferase under the control of a CMV promoter.

Formulation: AAV was produced in HEK293-AAV cells and is supplied in PBS-MK (PBS Magnesium-Potassium) buffer containing 0.01% Pluronic F68.

Purification: The purity of the AAV particles was confirmed to be greater than 90% by staining with One-Step Lumitein™ UV Protein Gel Stain (Biotium 21005-1L). Purity will vary with each lot; the exact value will be provided with each shipment.

Storage Stability: AAV is shipped with dry ice. For long-term storage, it is recommended to store AAV at -80°C. 

Supplied As: Two vials (50 µl x 2) of AAV at a titer ≥1 x 10¹² TU/ml. The titer is determined by qPCR and will vary with each lot; the exact value will be provided with each shipment.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS78452
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 78452
• Green Labware: No
• Package Unit: 50 µl x 2
• Quantity Unit: PAK