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Encompassing Amino Acids: 248-531
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in Kinase buffer containing 0.2 mM DTT using Abltide substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).
Description: Human Abelson murine leukemia viral oncogene homolog 1 (Abl-1) (GenBank Accession No. NM_007313), a.a. 248-531, with N-terminal His-tag, MW = 34.8 kDa, expressed in a Baculovirus-infected Sf9 cell expression system
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 200 mM imidazole, and 20% glycerol.
Genbank: NM_00731
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P00519
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cao, C., et al. 2003. J. Biol. Chem. 278(15):12961-7.
2. Yano, H., et al. 2000. J. Neurosci. Res. 59(3):356-364.Application Reference:LASP1 is a novel BCR-ABL substrate and a phosphorylation-dependent binding partner of CRKL in chronic myeloid leukemia (2014)
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