Note: Dry Ice fees will be extra-chargedProducts from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 1-2383(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A 25 µl ACC1 reaction is conducted in a buffer containing 30 mM HEPES (pH 7.4), 2 mM MgCl
2, 2 mM potassium citrate, 1 mM DTT, 100 µM ATP, 12 mM NaHCO
3 and 20 µM acetyl-CoA at 30°C for 45 min. ATP reduction is detected using ADP-Glo® Luminescent Assay Platform (Promega).
Description: Human ACC1 (acetyl-Coenzyme A carboxylase alpha transcript variant 1) (GenBank Accession No. NM_198834), a.a. 1-2383(end) with C-terminal FLAG and His-tags, MW = 270 kDa, expressed in Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT and variable FLAG peptide
Genbank: NM_198834
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-His-tags
Uniprot: Q13085
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sinilnikova, O.M. et al., Cancer Epidemiol. Biomarkers Prev. 16 (3), 409-415 (2007)
2. Olsen, J.V. et al., Cell 127 (3), 635-648 (2006) Application Reference(s):
A Cellular Assay for Inhibitors of the Fatty Acid Biosynthetic Pathway Using Scintillating Microplates (2015)
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