Description: This antibody reacts with placental alkaline phosphatase. There are at least four distinct but related alkaline phosphatases: intestinal, placental (PLAP), placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. PLAP is a tissue specific, trophoblast-derived, 70 kDa, glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, zinc metallo-glycoprotein that catalyzes the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. It is present in the placenta and serum of pregnant women and in high frequency in gynecological and testicular cancers and in lower frequency in other tumors. The three tissue-specific AP's in humans, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous. Non-tissue specific AP is found in kidney, liver and bone. This MAb binds equally well to all common allelic variants (S, F, FS and I) of PLAP and to some variants of AP from normal human testis. This MAb can be used as tracer antibody in ELISA to detect PLAP in serum of S, F, FS and I phenotypes. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: ALPP/516
Immunogen: Recombinant full-length human ALPP protein
Antibody Reactivity: Alkaline Phosphatase
Entrez Gene ID: 250
Antibody Application Notes: For coating for ELISA, order Ab without BSA/Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Optimal dilution and staining procedure for a specific application should be determined by user/Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
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