CloneID: 33F12
Antigen Long Description: The original antibody was generated by immunizing GIX+ mice with a keyhole limpet hemocyanin (KLH)-coupled diketone hapten.
Origin Pub PMID: 9405338
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody was raised against 1,3-diketone hapten, but it is capable of catalyzing the decarboxylation of structurally related β-keto acids.
Application Notes (Clone): This antibody efficiently catalyzes aldol and retro-aldol reactions and covalently binds 1,3-diketone hapten. The (kcat/KM)/kuncat is approximately 109 at pH 7.5 as measured by the consumption of substrates and production of aldol products (monitored using reversed-phase high-performance liquid chromatography (HPLC) and enzymatic assays) (Wagner et al., 1995; PMID: 8525368). This antibody efficiently catalyzed the decarboxylation of two hapten-related β-keto acids (marked 4 and 5 in scheme 1 in the publication) with significant rate enhancements (kcat/kuncat) of 4959 and 11,261, respectively (Björnestedt et al., 1996). The crystallization of the Fab version of this antibody revealed a binding pocket characterized by a narrow, elongated cleft, where the catalytic lysine residue is prominently located (Barbas III et al., 1997; PMID: 9405338).
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