CloneID: NAT-267
Antigen Long Description: The original antibody was generated by immunization of a llama (Llama glama) with the protein conjugate 15-DON-BSA in a TiterMax Classic adjuvant.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for mycotoxin 15-acetyldeoxynivalenol (15-AcDON). 15-Acetyldeoxynivalenol belongs to the class of type B trichothecene mycotoxins, generated by several Fusarium fungi. Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyldeoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock.
Application Notes (Clone): This antibody can bind 15-AcDON in a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) and fluorescence polarization assay (FPA). This antibody does not cross react with DON; 3-acetyldeoxynivalenol (3-AcDON); neosolaniol (NEO); diacetoxyscirpenol (DAS); and T-2 toxin. Surface plasmon resonance (SPR) analysis of soluble NAT-267 binding kinetics to immobilized 15-DON-Horse Radish Peroxidase (HRP) indicated a dissociation constant KD of 5microM (PMID: 18632156).
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