CloneID: S73-2
Antigen Long Description: This antibody was raised by immunizing mice with Kdo(2f8)[Kdo(2f4)]Kdo(2f4)Kdo(2f6)GlcNAc4P-(1f6)GlcNAcol-BSA and further immunizing them with Kdo(2f8)[Kdo(2f4)]Kdo-BSA.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for 2.4[2.8]2.4-linked Kdo tetrasaccharide. Which is a partial structure of chlamydial lipopolysaccharide (LPS).
Application Notes (Clone): To screen hybridomas for this antibody ELISA was performed on neoglycoconjugates and immunofluorescence was performed on cells infected with either C. trachomatis or C. psittaci. Surface plasmon resonance was performed on Kdo(2f8)Kdo(2f4)KdoBSA (164, 1650, and 2460 RUs), Kdo(2f4)Kdo(2f4)Kdo-BSA, Kdo(2f4)Kdo BSA and BSA(Sigma), as a reference protein using the mouse version of this antibody (Brooks et al, 2010; pmid:20000757).
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