CloneID: H2
Antigen Long Description: This antibody was generated by panning against purified virus using a antibody library from five different 5-week old white leghorn hens.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for the Blue tongue virus. It is the pathogen that causes blue tongue disease in ruminants.
Application Notes (Clone): To show that this antibody can detect virus particles, a sandwich ELISA was performed on blue tong virus, it showed a positive result (van Wyngaardt et al, 2004; pmid: 15059288). In a different study the scFv version of this antibody was omitted from an ELISA due to inconsistent results or low ELISA signal (Rakabe et al, 2011)