CloneID: IIB5
Antigen Long Description: The original antibody was generated by immunizing a BALB/c mouse intraperitoneally with BrdU conjugated to Bovine Serum albumin.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: The original antibody reacts with bromodeoxyuridine (BrdU) also when incorporated into nuclear DNA. Further, the antibody is known to cross-react with Iododeoxyuridine (IdU).
Application Notes (Clone): The binding characteristics of the antibody was determined in a radioimmunoassay using 3H-BrdUrd as a ligand, and the affinity constant was found to be 3.1 x 10^7 L/mol (Schutte et al., 1987; PMID: 3113895). This antibody was used for immunohistochemistry for the detection of bromodeoxyuridine (van Engeland et al., 1997; PMID: 9299167).
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