CloneID: 121SLE
Antigen Long Description: The original antibody was raised by immunizing a mouse with ten precipitin lines obtained after immunodiffusion using monoclonal antibody NS 19-9 and mucins isolated from an ovarian cyst of a OLe (a+b-) patient.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This antibody is specific for CA19-9.
Application Notes (Clone): The original version of this antibody was used for immunohistochemistry on the normal gastrointestinal mucosa of Le-positive individuals (Herrero-Zabaleta et al., 1987; PMID:2444292), and on endometrial tissue specimens obtained in different phases of the menstrual cycle (Hey and Aplin., 1996; PMID:8910004). This antibody was used for an ELISA on carbohydrate proteins (Manimala et al., 2007; PMID:17483136), and on MUC1 samples (Hey and Aplin., 1996; PMID:8910004). Furthermore this antibody was used for immunocytochemistry on HT 29 tumour cells (Köhler et al., 2009; PMID:20010946). The original version of this antibody was used for flow cytometry on ovarian cancer cell line SW626 and SCLC cell line DMS79 (Ragupathi et al., 2009; PMID:19190907).