CloneID: PL-2 scFv
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was raised by immunizing a BALB/c mouse with Human astrovirus-2.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted mouse scFv antibody, based on the original mouse scFv format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q82446
Specificity Statement: This antibody is specific for Capsid protein VP25 from Human astrovirus-2.
Application Notes (Clone): The structure of the original antibody was determined using x-ray crystallography. This antibody was used for a western blot on HAstV-2 capsid protein (Bogdanoff et al., 2016; PMID:27213181). The original antibody was used for an ELISA on purified Spike or GFP-Spike from HAstV-2 (Bogdanoff et al., 2017; PMID:27807234, Bogdanoff et al., 2018; PMID:29070688), and on purified Human astrovirus-2 (Sanchez-Fauquier et al., 1994; PMID:7514320). Further this antibody was used for surface plasmon resonance on Spike2 from HAstV-2. This antibody has shown that it is able to block the GFP-Spike2 binding to Caco-2 cells (Bogdanoff et al., 2017; PMID:27807234). This antibody was used for immunofluorescence on Human astrovirus-2 infected LLCMK2. This antibody was used for radioimmunoprecipitation on virus samples (Sanchez-Fauquier et al., 1994; PMID:7514320). This antibody was used for a neutralization assay by preincubating human astrovirus-2 with this antibody and then adding C2Bbe1 cells (Bogdanoff et al., 2018; PMID:29070688).
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