CloneID: 5B7
Antigen Long Description: The original antibody was isolated from a naïve human scFv phage library using multiple panning strategies including solid-phase panning with immobilized CD47 and solution-phase panning using biotinylated CD47.
Buffer Composition: PBS only.
Uniprot Accession No.: Q9Y275
Specificity Statement: This antibody binds human BAFF protein and cross reacts with mouse and cynomolgus monkey BAFF protein. B-cell-activating factor (BAFF/CD257) is a cytokine belonging to the tumour-necrosis factor (TNF) family. This cytokine binds to TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF is a survival factor for B cells that controls B-cell maturation. It is also involved in the stimulation of B- and T-cell function and the regulation of humoral immunity.
Application Notes (Clone): This antibody is capable of reacting with both soluble and membrane bound human, mouse and cynomolgus monkey BAFF/ BLyS protein. The binding ability of this antibody to membrane bound CHO-human BLys, HEK293-mouse BLyS and CHO-cynomolgus monkey BLyS protein was determined using flow cytometry. This antibody is capable of blocking the interaction between human BAFF and BR3. This antibody was also capable of neutralizing BLyS-mediated B-cell proliferation. The binding affinity characterization of this antibody was done using SPR. It was reported that the original IgG antibody binds human and mouse BLyS with a binding affinity of Kd= 4X10^(-10) M and Kd= 1.45X10^(-11) M respectively (US20170101466).
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