CloneID: CA17.2A12
Antigen Long Description: The original antibody was generated by immunizing mice with the T cell receptor complex (TCR/CD3) isolated from canine thymocytes.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Specificity Statement: This bispecific antibody is specific for canine TCR-2 (TCR-αβ) and CD3.
Application Notes (Clone): This antibody was used as an immune phenotype marker in flow cytometric (FC) analyses of peripheral blood lymphocytes (PBL) in dogs to identify T cells (specifically, CD3) (Helfand et al., 1995; PMID: 7605993). Later, it was used for immunophenotyping by FC to identify T-cell antigen CD3 in dogs with lymphocytosis (McDonough et al., 2000; PMID: 11105953) and as a T-lymphocyte marker in blood and bone marrow samples from dogs with hematologic disorders (Weiss, 2001; PMID: 11817066). It was also used in FC analysis of various cell populations, including peripheral blood and lymph nodes, to identify CD3
+ T cells in a study on apoptosis (Pappalardoa et al., 2001; PMID: 11730925). Most recently, it was used to stain leukocytes for analysis by FC as part of a Canine leukocyte suppression assay (LSA) (Amorim et al., 2020; PMID: 32698861), to identify CD3 in fine-needle aspirates of canine nodal lymphomas (Rigillo et al., 2021; PMID: 33759339), and to stain CD3 on canine peripheral blood mononuclear cells (PBMC) (Ruiz et al., 2022; PMID: 36217551). Additionally, this antibody was used to detect CD3 in immunohistology studies (specifically IHC) of the liver and spleen in dogs treated with rcGM-CSF (Nash et al., 1995; PMID: 7655007). This antibody was fixed to magnetic beads and used for the activation of canine peripheral blood lymphocytes (Szopa et al., 2021; PMID: 33679741). If you are interested in canine bispecific T-cell engagers, please
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