CloneID: OX-68
Heavy Chain modification: Fc Silent™
Antigen Long Description: This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously.
Buffer Composition: PBS only.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: P05540
Specificity Statement: This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69.
Application Notes (Clone): This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50% reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4.
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