Anti-CD48 (OX-45)

Anti-CD48 [OX-45], Recombinant, IgG2b kappa, Rat

CloneID: OX-45

Antigen Long Description: OX-45 was prepared by immunizing BALB/c mice with rat T blasts prepared by stimulating purified T helper (TH) cells with allogeneic irradiated rat spleen cells.

Buffer Composition: PBS only.

Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Uniprot Accession No.: P10252

Specificity Statement: OX-45 binds specifically to CD48 on rat thymocytes, T and B cells and endothelium but not LFA.3. Directly competes with CD46 for binding the target epitope. CD48 is a CD2 ligand and is expressed on the surface of all hematopoietic cells and endothelium. May be involved in the facilitation of interactions between activated lymphocytes. Likely to be involved in T cell activation.

Application Notes (Clone): Describes the use of OX-45 in blocking the primary mixed lymphocyte response (MLR)and the secondary response of T lymphocytes to the soluble antigen DNP-BGG. It also showed that OX-45 did not have any effect on the cytotoxic activity of specific (CTL) or non-specific (NK) killer cells or on proliferative responses stimulated by lectins or oxidative mitogenesis.

• SKU: ABAAb00571-8.1-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab00571-8.1-BT
• Package Unit: 1 mg
• Quantity Unit: STK
• Reactivity: Rat (Rattus)
• Clonality: Recombinant
• Application: Flow Cytometry, Blocking
• Isotype: IgG2b kappa
• Host: Rat