Anti-CD86 (OX-48)

Anti-CD86 [OX-48], Recombinant, IgG2b kappa, Rat
SKU
ABAAb00569-8.4
Packaging Unit
200 μg
Manufacturer
Absolute Antibody

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CloneID: OX-48

Heavy Chain modification: Fc Silent™

Antigen Long Description: OX-48 was prepared by immunizing mice with activated rat T cell blasts

Buffer Composition: PBS with 0.02% Proclin 300.

Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7

Uniprot Accession No.: Q62810

Specificity Statement: OX-48 binds specifically to CD86 targets which are present on subsets of T and B blasts, spleen macrophages and polymorphs, peritoneal macrophages, dendritic cells. OX48 does not appear to bind the same epitope of CD86 as 24F (Stephens et al. 2004). CD86 is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes - expression is rapidly upregulated by B cell specific stimuli. CD86 acts as a ligand for CD28 and CTLA-4. CD86 works in tandem with CD80 to prime T cells. 2 splice isoforms exist.

Application Notes (Clone): OX-48 blocks IL-2 production and proliferation of T cells (except when in exponential phase) (Samoza et al. 1990). The antibody can also be used for FC (Stephens et al. 2004), FACS and IPs. Ox-48 has been shown to be able to block binding of CD86 with its ligand CD28.
More Information
SKU ABAAb00569-8.4
Manufacturer Absolute Antibody
Manufacturer SKU Ab00569-8.4
Package Unit 200 μg
Quantity Unit STK
Reactivity Rat (Rattus)
Clonality Recombinant
Application Immunofluorescence, Flow Cytometry
Isotype IgG2b kappa
Host Rat
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