CloneID: 10D2A10F9
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with CD99.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P14209
Specificity Statement: The antibody is specific for CD99. CD99 is a type I transmembrane glycoprotein with a molecular weight of 32kDa. CD99 is involved in the process of cell adhesion and apoptosis. It is produced by thymocytes and T cells. It plays an important role in the interaction between cells and extracellular matrix and between cells and cells, and affects the development and metastasis of tumor cells.
Application Notes (Clone): The antibody specifically detected CD99 by Western blot analysis. The antibody 10D2A10F9 and the commercial antibody O13 were used to stain ovarian granulosa cell tumor and primitive neuroectodermal tumor. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 10D2A10F9 was higher than the commercial antibody. The antibody and the commercially available antibody O13 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113929783A).
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