CloneID: scFvB9
Heavy Chain modification: ScFv
Antigen Long Description: The original antibody was isolated from Tomlinson phage display antibody library by screening against coat protein of Chickpea chlorotic dwarf virus (CpCDV) expressed in Escherichia coli strain Rosetta (DE3).
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human scFv antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: A0A0H4SQY8
Specificity Statement: This antibody binds the coat protein of Chickpea chlorotic dwarf virus (CpCDV). CpCDV is a monopartite, single-stranded circular DNA virus in the genus Mastrevirus and family Geminiviridae. It is transmitted by Orosius albicinctus in a circulative (persistent) and nonpropagative manner. It is the causative agent for Chickpea stunt disease (CSD) that is a threat to chickpea production leading to yield losses of 75⁻95%. Symptoms of CSD include very small leaves, intense discoloration (yellowing (kabuli type) and reddening (desi type)), and bushy stunted appearance of the plant.
Application Notes (Clone): The affinity characterization of this antibody for Chickpea chlorotic dwarf virus (CpCDV) was done using ELISA. This antibody reacted with recombinant coat protein of CpCDV in a western blot (PMID: 32970278).
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