CloneID: P02
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with C-reactive protein extracted from human plasma.
Buffer Composition: PBS only.
Uniprot Accession No.: P02741
Specificity Statement: This antibody reacts with human C reactive protein, which is a pentameric protein found in blood plasma. It is an acute phase reactive protein of hepatic origin. It is an important defense molecule for the body and its plasma concentration increases sharply during tissue damage, inflammation and bacterial infection. It promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Application Notes (Clone): Indirect ELISA was used for the primary screening of the hybridomas showing selective binding for human CRP. Spicy root labelled scFv P02 was used for the identification of natural CRP extracted from denatured and non-denatured blood in a western blot assay. The combination of P02 and P20 antibody was also used in the double antibody sandwich detection system consisting of P20 as the coating antibody and P02 as the labeled antibody to be used in the colloidal gold detection platform for the detection of natural CRP. These antibodies were also used in the preparation of human C-reactive protein colloidal gold test card (CN106596976).
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