CloneID: V44D7
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with CXCR-3 expressing NSO cells. The humanized version of the antibody was created by grafting CDRs of the original murine antibody onto human framework regions.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P49682
Specificity Statement: This antibody binds human CXCR3. This protein acts as a receptor for C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway. Isoform 2 human mediates the proliferation, survival and angiogenic activity of microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway. Some of the disorders associated with CXCR3 signaling include diabetes mellitus type 1 and psoriasis.
Application Notes (Clone): The binding characterization of this antibody to human CXCR3 was done using competitive ELISA. This antibody is capable of inhibiting binding of 125I-labeled IP-10 and -I-Tac to primary Th1 cells. This antibody can be used for the study of expression of CXCR3 on the surface of Th1 cells using flow cytometry. This antibody also inhibited Th1 cell migration mediated by CXCR3 chemokines (US8435522).
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