CloneID: 19A11
Antigen Long Description: The original antibody was generated by immunizing mice with purified ECad1-5 protein.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P12830
Specificity Statement: This antibody recognizes the N-terminal extracellular cadherin repeat domain 1 (EC1) of the human E-cadherin. This antibody also cross reacts with canine E-cadherin. Cadherins are calcium-dependent cell adhesion proteins. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. E-cadherins are reported to play an important role in cancer metastasis.
Application Notes (Clone): The initial screeding of E-Cadherin–positive hybridoma supernatants for binding to live Colo 205 cells was done using an ELISA. Activating antibody 19A11 stimulated the adhesion of Colo 205 cells to pure E-cadherin substrate (PMID: 22513089). This antibody is also capable of recognizing denature human E-cadherin in a western blot. This antibody also inhibited the formation of epithelial tubules, causing the cells to remain as round epithelial cysts. This antibody also enhanced the adhesion of HGF-treated MDCK cells to E-cadherin when analyzed with the flow adhesion assay (PMID: 22513089). This antibody slowed the rate of wound closure in both normal culture medium and HGF-treated cultures (PMID: 22513089). This antibody is an E-cadherin activating antibody which stimulates the apoptosis of circulating tumor cells (PMID: 32127478). This antibody was used in determination of expression of E-cadherin on low-adherent Colo205 cells using flow cytometry (PMID: 27582386).
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