CloneID: WD11F
Antigen Long Description: An immune phage display library derived from the peripheral blood lymphocytes of a llama that had been immunized with an equine vaccine that includes killed WEEV (West Nile Innovator + VEWT) was generated. The antibody was selected by panning the library against recombinant envelope (E2/E3E2) protein from WEEV.
Buffer Composition: PBS only.
Specificity Statement: The antibody is specific for E2/E3E2 envelope protein.Two envelope proteins, glycoproteins E1 and E2, associate as trimers of E1-E2 dimers on the viral surface, and make up 80 spikes on the viral surface.
Application Notes (Clone): The binding affinity of the VHH fragment for the WEEV E3E2 recombinant protein was measured by surface plasmon resonance (Kd= 7.2 ×10^−9 M). The ability of the antibody to detect the recombinant protein in a MagPlex sandwich immunoassay was confirmed (Liu et al., 2018; PMID: 31544894).
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