CloneID: 93k
Antigen Long Description: The original hybridoma was generated using the trioma method of Ostberg et al. (1983), wherein an immunized human B cells from an adult sero-positive for VZV was fused with a xenogenic mouse-human cell that was produced by fusing the drug resistant mouse cell line SP-2 with normal human peripheral lymphocytes called the SPAZ-4 cell line. This hybridoma generated an antibody 93K that was capable of neutralizing VZV in absence of complement.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P09257
Specificity Statement: This antibody targets the domain IV region of envelope glycoprotein B of the Varicella zoster virus (VZV) also known as the human herpesvirus 3 (HHV-3, HHV3) or Human alpha herpesvirus 3. This DNA virus from the herpes virus group can infect humans causing chickenpox in children and shingles (herpes zoster) in adults. Varicella is characterized by a maculopapular, vesicular rash that can be pruritic and evolves into dried crusts (scabs) over a 3- to 7-day period. Chicken pox is considered contagious beginning 1-2 days before rash onset until all lesions have crusted (scabbed).
Application Notes (Clone): This antibody is capable of detecting full length VZV glycoprotein B (gB) in a western blot. It was reported that this antibody could not bind to the gB R592A, I594A, or 589AAA594 mutants in western blots and binding was reduced but not abolished for the Q596A, N597A, or 596AA597 mutants (PMID: 32811830). This antibody is capable of neutralizing MRC-5 human primary fibroblast cells infected with K.McC. clinical strain of VZV virus (US5506132). It also reduced gB/gH-gL-mediated membrane fusion by 95% in the stable reporter fusion assay (SRFA) in the absence of other viral proteins (PMID: 32811830). This antibody was also used for the immunoprecipitation of gB β23 mutants and gB/gH-gL fusion complex (PMID: 32811830).
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