CloneID: Fab-RC
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was raised from the white blood cells of a human individual immunized with zoster vaccine.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG format, created for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: Q775J3; Q9J3N1
Specificity Statement: This antibody is specific for the combination of gH and gL and does not detect the proteins in isolation.
Application Notes (Clone): The original antibody was used for surface plasmon resonance on VZV gHgL. This antibody was used for a neutralization assay. Here is showed it was able to inhibit gB/gHgL-mediated membrane fusion. The structure of this antibody was determined using x-ray crystallography (Xing et al., 2015; PMID:25918416). This antibody was used for immunohistochemistry on fixed and unfixed VZV-infected and control HFL cells. This antibody was also used for a plaque reduction assay on VZV-infected HFL cells. Further this antibody was used for immunoprecipitation on VZV-infected and uninfected HFL cells (Birlea et al., 2013; PMID:23077312).
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