Anti-envelope protein (ZIKVEF3S06)

Anti-envelope protein [ZIKVEF3S06], Recombinant, scFv fragment (His)

CloneID: ZIKVEF3S06

Heavy Chain modification: ScFv

Antigen Long Description: The original antibody was generated by immunizing chicken with ZIKV envelope protein followed by antibody library construction and phage display-based selection.

Buffer Composition: PBS only.

Specificity Statement: The antibody binds to the E protein of Zika virus. The envelope protein forms a dimer on the smooth surface of the mature virus, and participates in receptor binding and fusion to the host.

Application Notes (Clone): The specificity of the scFv format of the antibody was confirmed by ELISA analysis. The antibody detected the recombinant E protein by western blot analysis. The scFv format recognized the E protein in a transfected Vero cell by flow cytometry. Competitive inhibition assay was employed to calculate the percentage of inhibition and the dissociation constant of the antibody against the E protein (inhibition of 50% = 77.42 ug/mL and Kd= 4.55 *10^-6 M).The antibody has diagnostic or therapeutic potential for ZIKV (PMID: 31940993).

• SKU: ABAAb03414-30.11-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03414-30.11-BT
• Package Unit: 500 μg
• Quantity Unit: STK
• Reactivity: Virus
• Clonality: Recombinant
• Application: Western Blotting, ELISA, Flow Cytometry, Inhibition
• Isotype: scFv fragment (His)