CloneID: R15
Antigen Long Description: This original antibody was raised by screening B cells from a Rift Valley fever patient and sequencing the VH and VL antibody regions.
Buffer Composition: PBS with 0.02% Proclin 300.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P03518
Specificity Statement: This antibody is specific for the DI domain, including residues 173-TQEDATCKPVTYA-185, P273, K274, M287, and 290-ASLKK-294 of rift valley fever glycoprotein N
Application Notes (Clone): The original version of this antibody was used for an ELISA on rift valley fever Gn and Gc. Further this antibody was used for flow cytometry on HEK293T cells transiently transfected with plasmids of pEGFP-M, pEGFP-Gn, pEGFP-Gc or pEGFP-Gn mutants. This antibody was used for a blocking assay, the rift valley virus was preincubated with this antibody and the mixture was then added to Vero cells. This antibody was used for a western blot on its linear epitope. This antibody was used for surface plasmon resonance on rift valley fever Gn and Gc. A neutralization assay with this antibody was performed using flow cytometry on rift valley virus and Vero cells. The antibodies were added pre-infection. This antibody was used for an in vivo assay. Mice were infected with the rift valley virus. Then post infection they were given this antibody. This lead to all of the mice surviving, where the control group all died. Finally the structure of this antibody was determined using x-ray crystallography (Wang et al., 2019; PMID:30936489).
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