CloneID: 4B5E6D7
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated by immunizing BALB/c mice with EP-CAM.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P16422
Specificity Statement: The antibody is specific for EP-CAM. EP-CAM is a transmembrane glycoprotein mediating Ca²⁺-independent homotypic cell–cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. It may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. It plays a role in embryonic stem cells proliferation and differentiation and it up-regulates the expression of FABP5, MYC and cyclins A and E.
Application Notes (Clone): The antibody 4B5E6D7 and the commercial antibody MOC-31 were used to stain lung squamous cell carcinoma, lung adenocarcinoma, cervical cancer, colorectal carcinoma, duodenum and rectal tissues. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 4B5E6D7 was higher than the commercial antibody. The antibody and the commercially available antibody MOC-31 were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN110922485B).
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