Anti-F protein (3B1)

Anti-F protein [3B1], Recombinant, Fab fragment kappa, Human

CloneID: 3B1

Heavy Chain modification: His-Tagged

Antigen Long Description: A synthetic, fully human antibody library was used for panning against RSV prefusion and post fusion F proteins. The original antibody against RSV F protein was discovered following phage library panning and characterized.

Buffer Composition: PBS only.

Uniprot Accession No.: P03420

Specificity Statement: The original antibody is specific for post fusion RSV F protein (amino acids D385, F387 and N388). F-protein plays a pivotal role in the pathogenesis of RSV by mediating the fusion between the viral and the host cell membrane. F protein exists in two distinct conformations: the metastable prefusion conformation and the stable post fusion conformation.

Application Notes (Clone): The specificity of the Fab and IgG1 format of the antibody was confirmed by ELISA analysis (EC50 value for IgG1 format at 3.6 ng/mL). The binding affinity of the Fab fragment to post fusion form of F was measured by surface plasmon resonance (Kd= 68 nM).

• SKU: ABAAb03524-10.29-BT
• Manufacturer: Absolute Antibody
• Manufacturer SKU: Ab03524-10.29-BT
• Package Unit: 500 μg
• Quantity Unit: STK
• Reactivity: Virus
• Clonality: Recombinant
• Application: ELISA, Super-Resolution Microscopy
• Isotype: Fab fragment kappa
• Host: Human