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Applications: Useful for studying the binding of FcGR3A and EGFR in ELISA and in cellular assays.
Assay Conditions: Functional validation: The antibody was validated by measuring anti-FcGR3A-anti-EGFR binding to FcGR3A and EGFR antigens in ELISA assay. The FcGR3A protein (BPS Bioscience #79013) and EGFR protein were coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of purified anti-FcGR3A-anti-EGFR antibody (BPS Bioscience #101535), serially diluted in Blocking Buffer 2, was added for 30 minutes at room temperature. After 3 more washes, the plate was incubated with Goat Anti-Human IgG Fc (HRP) (Abcam #ab97225), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.
Description: Anti-FcGR3A-anti-EGFR bispecific IgG antibody, which recognizes FcGR3A (also known as CD16A) and EGFR antigen binding proteins. The amino acid sequences correspond to the sequences from the bispecific EGFR/FcGR3 antigen-binding protein patent WO2019175368A1. This bispecific antibody has been tested for specific binding affinity to purified human FcGR3A and EGFR recombinant protein.
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Host Cell Line: HEK293
Purity: ≥90%
Storage Stability: At least 12 months at -80°C.
Uniprot: FcGR3A: P08637; EGFR: P00533
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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