CloneID: L19 (Radretumab)
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was generated using phage display from a human antibody library.
Buffer Composition: PBS with 0.02% Proclin 300.
Chimeric Use Statement: This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Available Custom Conjugation Options: AP, HRP, Fluorescein, APC, PE, Biotin Type A, Biotin Type B, Streptavidin, FluoroProbes 647H, Atto488, APC/Cy7, PE/Cy7
Uniprot Accession No.: P02751
Specificity Statement: This antibody is specific for human fibronectin; it binds an epitope within the ED-B domain.
Application Notes (Clone): The original version of this antibody (human scFv) was determined to bind human fibronectin at the ED-B domain with a Kd of 54 pM using SPR. Furthermore, the antibody forms a stable complex with the fluorescently labeled ED-B and exhibits a long half-life when complexed with it (Pini et al., 1998; PMID: 9705314). This antibody was used for ELISA on biotinylated ED-B. Furthermore, it was used for a radioimmunoassay conducted on mice with subcutaneously implanted murine F9 teratocarcinoma, and for immunohistochemistry and Immunofluorescence performed on the corneas of New Zealand white rabbits (US8097254). Various formats of this antibody were used in an ELISA on the ED-B domain of FN (Borsi et al, 2002; pmid:12353237). This antibody was used for immunohistochemistry on tissues from patients with HNSCC (Birchler et al., 2003; PMID: 12838025). A radiolabeled version of this antibody was used for SPECT/CT on patients with SCC (Birchler et al., 2007; PMID: 17418248).
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