CloneID: HENV-26
Heavy Chain modification: Fc Silent™
Antigen Long Description: The original antibody was isolated from an immune human individual.
Buffer Composition: PBS only.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Uniprot Accession No.: Q9IH62; F4YH71; H6V875; O89343
Specificity Statement: The antibody is specific for RBP of HeV and NiV. The antibody epitope overlaps with the receptor binding sites of RBP.
Application Notes (Clone): The specificity of the original format of the antibody for recombinant RBP head domain proteins from HeV, NiVM, or NiVB was confirmed by ELISA analysis (EC50 = 0.14,0.09 and 0.07 ug/mL respectively). The antibody could neutralize HeV, NiVB and NiVM in In vitro neutralization assays (IC50= 0.07, 0.03 and 0.04 ug/mL respectively). The antibody could bind to the full-length RBPs by flow cytometry. The EC50 values for binding of the antibody ranged from 325 to 343 ng/mL for binding to HeV, NiVM, or NiVB. The kinetics of binding of the antibody to RBPs was measured on a biosensor to determine affinity (KD= 2.9, 2.2, or 1.0 nM for HeV, NiVM, or NiVB, respectively). The crystal structures of Fab fragment of the antibody in complex with both HeV-RBP and NiV-RBP were determined. The antibody protected ferrets in lethal models of infection with NiV (Dong et al., 2020; PMID: 33306954).
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